- 13 Sep 2025, Markinch awarded 3 points as Balgonie Scotia forfeited
11th October, 2025
- Bridge of Earn - Balgonie Scotia
- Kinross Colts - Bowhill Rovers
- Markinch - Burntisland United
- Pittenweem Rovers - St Andrews Amateurs
4th October, 2025
- Balgonie Scotia 4 - 3 Kinross Colts
27th September, 2025
- Balgonie Scotia 5 - 0 St Andrews Amateurs
- Bowhill Rovers 6 - 1 Fife Thistle
- Kinross Colts 2 - 3 Markinch
- Leslie Hearts 1 - 3 Pittenweem Rovers
- Leven United 0 - 2 Greig Park Rangers
- Lochgelly United 4 - 3 Bridge of Earn
- The Swifts 3 - 1 Burntisland United
20th September, 2025
- Balgonie Scotia 1 - 0 Leven United
- Bowhill Rovers 3 - 1 St Andrews Amateurs
- Bridge of Earn 2 - 1 Fife Thistle
- Burntisland United 3 - 2 Pittenweem Rovers
- Greig Park Rangers 6 - 0 Leslie Hearts
- Lochgelly United 2 - 4 Cupar Hearts
- Markinch 4 - 1 The Swifts
13th September, 2025
- Burntisland United 2 - 0 Bridge of Earn
- Cupar Hearts 2 - 2 Leven United
- Kinross Colts 6 - 1 Pittenweem Rovers
- Markinch - Balgonie Scotia * match awarded to Markinch
- St Andrews Amateurs 1 - 4 Fife Thistle
- The Swifts 2 - 2 Lochgelly United
6th September, 2025
- Bowhill Rovers 4 - 1 The Swifts
- Leven United 6 - 2 Lochgelly United
30th August, 2025
- Bowhill Rovers 0 - 3 Leven United
- Bridge of Earn 4 - 0 St Andrews Amateurs
- Burntisland United 1 - 2 Kinross Colts
- Fife Thistle 1 - 5 Cupar Hearts
- Leslie Hearts 6 - 2 Balgonie Scotia
- Lochgelly United 1 - 2 Markinch
- Pittenweem Rovers 0 - 5 Greig Park Rangers
26th August, 2025
- Balgonie Scotia 2 - 3 Bowhill Rovers
- Cupar Hearts 12 - 0 Pittenweem Rovers
- Greig Park Rangers 7 - 4 Bridge of Earn
- Lochgelly United 2 - 0 Burntisland United
- Markinch 4 - 2 Leslie Hearts
- St Andrews Amateurs 5 - 1 Kinross Colts
- The Swifts 2 - 2 Fife Thistle
23rd August, 2025
- Balgonie Scotia 0 - 3 The Swifts
- Bowhill Rovers 0 - 3 Markinch
- Bridge of Earn 0 - 3 Cupar Hearts
- Burntisland United 6 - 2 St Andrews Amateurs
- Fife Thistle 2 - 6 Leven United
- Kinross Colts 1 - 10 Greig Park Rangers
- Leslie Hearts 1 - 2 Lochgelly United
19th August, 2025
- Balgonie Scotia 2 - 1 Fife Thistle
- Cupar Hearts 11 - 1 Kinross Colts
- Greig Park Rangers 6 - 1 St Andrews Amateurs
- Leslie Hearts 1 - 4 Burntisland United
- Leven United 0 - 2 Pittenweem Rovers
- Lochgelly United 2 - 1 Bowhill Rovers
- The Swifts 3 - 0 Bridge of Earn
16th August, 2025
- Bowhill Rovers 4 - 1 Leslie Hearts
- Bridge of Earn 3 - 4 Leven United
- Burntisland United 1 - 6 Greig Park Rangers
- Fife Thistle 1 - 0 Markinch
- Kinross Colts 0 - 4 The Swifts
- Pittenweem Rovers 1 - 4 Balgonie Scotia
- St Andrews Amateurs 0 - 4 Cupar Hearts
